Oh, today’s thirteen, happy birthday, YUUKA!
Yesterday afternoon, we did our graduation theses’ commencement report.
Of the lab which I applied in, in all, there were four professions and teachers who instruct us. For each tutor could instruct five students, the students’ number was much. Therefore, our report time was shrunk to about 5 minuets.
A little report re-shrink version of mine:
Of the lab which I applied in, in all, there were four professions and teachers who instruct us. For each tutor could instruct five students, the students’ number was much. Therefore, our report time was shrunk to about 5 minuets.
A little report re-shrink version of mine:
[TITLE] Construction of Expressing Vector for Antifungal Polypeptide
[MEANING] Antifungal peptide (AFP), is a small protein or peptide, which induced by fungal infection, and is produced by diverse organisms, include bacteria, fungi, plants, insects, and mammals. AFPs have potent activity to defend fungal infection. Its active mechanism is not the same to antibiotics, so that most fungi would not have restraint. To clone AFPs’ genes and combine them into expressing vectors, so we can product AFPs faster and better.
[RESEARCH SUBJECT] Isolate RNAs from Brassica nupus (rape) and some other plants; design a pairs of specific primers for RT-PCR; construct a expression vector (use PET30a plasmid); transform into E. coli and follow inspections.
[STEP LINE]
- Isolation of plant (rape & radish) RNAs
- Designing of specific primers
- RT-PCR to amplify AFPs’ cDNAs
- Electrophoresis and reclaiming purpose band
- Cloning to T-vector, sequencing
- Cleaving of T-vector and purpose expression vector (PET30a) by restriction endonucleases
- Electrophoresis and reclaiming
- T4 linkage of fragments and plasmids
- Transforming into E. coli
- Selecting recombine colonies and verifying by PCR
- Expressed proteins’ inspection
[REFERENCE] Antifungal Proteins, CLAUDE P. SELITRENNIKOFF, APPLIED AND ENVIRONMENTAL MICROBIOLOGY. | July 2001: 2883–2894.
[MEANING] Antifungal peptide (AFP), is a small protein or peptide, which induced by fungal infection, and is produced by diverse organisms, include bacteria, fungi, plants, insects, and mammals. AFPs have potent activity to defend fungal infection. Its active mechanism is not the same to antibiotics, so that most fungi would not have restraint. To clone AFPs’ genes and combine them into expressing vectors, so we can product AFPs faster and better.
[RESEARCH SUBJECT] Isolate RNAs from Brassica nupus (rape) and some other plants; design a pairs of specific primers for RT-PCR; construct a expression vector (use PET30a plasmid); transform into E. coli and follow inspections.
[STEP LINE]
- Isolation of plant (rape & radish) RNAs
- Designing of specific primers
- RT-PCR to amplify AFPs’ cDNAs
- Electrophoresis and reclaiming purpose band
- Cloning to T-vector, sequencing
- Cleaving of T-vector and purpose expression vector (PET30a) by restriction endonucleases
- Electrophoresis and reclaiming
- T4 linkage of fragments and plasmids
- Transforming into E. coli
- Selecting recombine colonies and verifying by PCR
- Expressed proteins’ inspection
[REFERENCE] Antifungal Proteins, CLAUDE P. SELITRENNIKOFF, APPLIED AND ENVIRONMENTAL MICROBIOLOGY. | July 2001: 2883–2894.